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Image Search Results
Journal: Molecular cell
Article Title: Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
doi: 10.1016/j.molcel.2020.01.022
Figure Lengend Snippet: Figure 6. Inhibition of TLR2-Induced Inflammation by DRD5 Activation Requires the ARRB2/PP2A Signaling Complex (A) Left: qRT-PCR analysis of Il6 and Tnf mRNA expression in WT and Arrb2/ BMDMs treated as indicated for 6 h. ELISA of IL-6 and TNF-a in su- pernatants from WT and Arrb2/ BMDMs treated as indicated for 18 h. (B) Immunoblot analysis of p-IkBa, p-p38, and ARRB2 in lysates of WT and Arrb2/ BMDMs treated as indicated. (C) Left: qRT-PCR analysis of Il6 and Tnf mRNA expression in PP2Acf/f and PP2Acf/fLysM-Cre BMDMs treated as indicated for 6 h. Right: ELISA of IL-6 and TNF-a in supernatants from PP2Acf/f
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-IkBa Cell Signaling Technology Cat#9246; RRID: AB_2267145 p-JNK Cell Signaling Technology Cat#9251; RRID: AB_331659 p-p38 Cell Signaling Technology Cat#9211; RRID: AB_331641 p-IKKa/b Cell Signaling Technology Cat#2697; RRID: AB_2079382 JNK Cell Signaling Technology Cat#9252; RRID: AB_2250373 p38 Cell Signaling Technology Cat#9212; RRID: AB_330713 IKKb Cell Signaling Technology Cat#2370; RRID: AB_2122154 MYC Cell Signaling Technology Cat#2276; RRID: AB_331783 FLAG Sigma-Aldrich Cat#F3165; RRID: AB_259529 actin Sigma-Aldrich Cat#A1978; RRID: AB_476692 HA Covance Cat#16B12; RRID: AB_10063630 TRAF6 Santa Cruz Cat#sc-7221; RRID: AB_793346 IBA1 abcam Cat#019-19741; RRID: AB_839504 DRD1 Hangzhou HuaAn Biotechnology Cat#ET1703-45; RRID: AB_ 2815007 DRD2 Bioss Cat#bs-20730R; RRID: AB_ 2815008 DRD3 Affinity Cat#DF10212; RRID: AB_ 2815010 DRD4 Bioss Cat#bs-1746R; RRID: AB_
Techniques: Inhibition, Activation Assay, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot
Journal: Molecular cell
Article Title: Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.
doi: 10.1016/j.molcel.2020.01.022
Figure Lengend Snippet: Figure 7. DRD5-ARRB2-PP2A Signaling Acti- vation Prevents S. aureus-Induced Sepsis and Meningitis (A) Survival of WT, Drd5/, Arrb2/, and PP2Acf/f
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies p-IkBa Cell Signaling Technology Cat#9246; RRID: AB_2267145 p-JNK Cell Signaling Technology Cat#9251; RRID: AB_331659 p-p38 Cell Signaling Technology Cat#9211; RRID: AB_331641 p-IKKa/b Cell Signaling Technology Cat#2697; RRID: AB_2079382 JNK Cell Signaling Technology Cat#9252; RRID: AB_2250373 p38 Cell Signaling Technology Cat#9212; RRID: AB_330713 IKKb Cell Signaling Technology Cat#2370; RRID: AB_2122154 MYC Cell Signaling Technology Cat#2276; RRID: AB_331783 FLAG Sigma-Aldrich Cat#F3165; RRID: AB_259529 actin Sigma-Aldrich Cat#A1978; RRID: AB_476692 HA Covance Cat#16B12; RRID: AB_10063630 TRAF6 Santa Cruz Cat#sc-7221; RRID: AB_793346 IBA1 abcam Cat#019-19741; RRID: AB_839504 DRD1 Hangzhou HuaAn Biotechnology Cat#ET1703-45; RRID: AB_ 2815007 DRD2 Bioss Cat#bs-20730R; RRID: AB_ 2815008 DRD3 Affinity Cat#DF10212; RRID: AB_ 2815010 DRD4 Bioss Cat#bs-1746R; RRID: AB_
Techniques: